To evaluate the antitumor activity and antimetastatic effects of HDBT, studies were done experimentally.
The results were obtained as follows :
1. HDBT extracts didn’t show cytotoxicity against BALB/C mouse lung fibroblast cell.
2. In cytotoxicity against A549, SK-OV-3, B16-BL6 and HT1080 concentration inhibiting cell growth up to below 30% of control was recognized at 10-3g/ml of HDBT.
3. The concentration inhibiting adhesion of A549 and B16-BL6 to complex extracellular matrix up to below 30% of control was recognized at 10-3g/ml of HDBT.
4. In Inhibitory effect on activity of DNA topoisomerase I, the IC50 was shown 200-300 ㎍/ml of HDBT.
5. The T/C% was 137.9% in HDBT-treated group in S-180 bearing ICR mice.
6. In CAM assay, HDBT extracts inhibited angiogenesis significantly at 15 ㎍/egg concentration as compared with control.
7. In pulmonary colonization assay, a number of colonies in the lungs were decreased but insignificantly in HDBT-treated group as compared with control group.
8. In hematological changes in B16-BL6 injected C57BL/6, numbers of WBC were decreased significantly in HDBT-treated group but numbers PLT were increased insignificantly as compared with control.
From above results it was concluded that HDBT could be usefully applied for the prevention and treatment of cancer.
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